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| Name |
| Costa, Guilherme |
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| E-Mail |
| guilherme.mc.costa@gmail.com |
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| 1st Degree |
| Biology |
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| University (1st Degree) |
| Universidade de Lisboa |
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| About the PhD |
| Field of Research |
| Stem Cell Haematopoiesis |
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| Thesis Title |
| Exploring the role of SOX7 in the specification of the haemogenic endothelium |
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| Abstract |
| During vertebrate ontogeny the haemangioblast generates the first blood cells through an endothelial stage with haemogenic properties. Although several transcription factors have been implicated in the development of the... |
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| During vertebrate ontogeny the haemangioblast generates the first blood cells through an endothelial stage with haemogenic properties. Although several transcription factors have been implicated in the development of the haemangioblast, the precise mechanisms governing cell fate decisions towards the haemogenic endothelium remain largely unknown. Under defined conditions, mouse embryonic stem (mES) cells can be differentiated into haemangioblast-like progenitors that recapitulate early embryonic haematopoiesis. In this study, we used mES cells as a model to understand the role of SOX7, a transcription factor expressed transiently at the onset of haemangioblast differentiation. Blast colony cells over-expressing SOX7 sustained the expression of endothelial cell surface markers and showed a reduction in the capacity to generate mature haematopoietic cells. Interestingly, the ectopic expression of SOX7 in fully committed blood precursors reactivated a transcriptional programme reminiscent of a former endothelial-related stage. In particular, we demonstrate that SOX7 can bind and activate the promoter of the endothelial-specific adhesion molecule VE-Cadherin. Altogether, our findings suggest that SOX7 is involved in the specification of the haemogenic endothelium, providing new clues to understand the molecular pathways that drive early embryonic haematopoiesis. |
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| Supervisor(s) |
| Dr. Valerie Kouskoff |
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| University |
| University of Manchester |
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| Laboratory |
| Paterson Institute for Cancer Research |
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| City |
| Manchester |
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| Country |
| UK |
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| Date of Thesis Defence |
| 2011-07-13 |
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| After the PhD (Current Situation) |
| Position |
| Dean's Prize (early Career Researcher Development Scheme) |
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| Project |
| mRNA localisation in angiogenesis |
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| Institution |
| University of Manchester |
| View Institution website |
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| City |
| Manchester |
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| Country |
| UK |
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| Relevant Publications |
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Guilherme Costa, Andrzej Mazan, Arnaud Gandillet, Stella Pearson, Georges Lacaud, and Valerie Kouskoff
SOX7 regulates the expression of VE-cadherin in the haemogenic endothelium at the onset of haematopoietic development
Development 2012;139 1587-1598 |
| View Publication |
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| Costa G, Harrington KI, Lovegrove HE, Page DJ, Chakravartula S, Bentley K, Herbert SP. Asymmetric division coordinates collective cell migration in angiogenesis, Nature Cell Biology 2016, 18: 1292-1301. |
| Publications |
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Costa G, Kouskoff V, Lacaud G.
Origin of blood cells and HSC production in the embryo.
Trends Immunol. May, 2012 Volume 33, Issue 5 |
| View Publication |
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Christophe Lancrin, Milena Mazan, Monika Stefanska, Rahima Patel, Monika Lichtinger, Guilherme Costa, Özge Vargel, Nicola K. Wilson, Tarik Möröy, Constanze Bonifer, Berthold Göttgens, Valerie Kouskoff and Georges Lacaud
GFI1 and GFI1B control the loss of endothelial identity of hemogenic endothelium during hematopoietic commitment
Blood 2012;120 314-322 |
| View Publication |
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| Perez-Campo FM, Costa G, Lie-a-Ling M, Kouskoff V, Lacaud G. The MYSTerious MOZ, a histone acetyltransferase with a key role in haematopoiesis. Immunology 2013; 139(2):161-5. |
| View Publication |
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| Perez-Campo FM, Costa G, Lie-A-Ling M, Stifani S, Kouskoff V, Lacaud G. MOZ-mediated repression of p16INK4a is critical for the self-renewal of neural and hematopoietic stem cells. Stem Cells 2013 |
| View Publication |
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| Stefanska M, Costa G, Lie-A-Ling M, Kouskoff V, Lacaud G. Smooth muscle cells largely develop independently of functional hemogenic endothelium. Stem Cell Res. 2014 Jan;12(1):222-32 |
| View Publication |
[more publications]
| More Publications |
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| Lilly AJ, Costa G, Largeot A, Fadlullah MZ, Lie-A-Ling M, Lacaud G, Kouskoff V. Interplay between SOX7 and RUNX1 regulates hemogenic endothelial fate in the yolk sac, Development 2016, 143: 4341-4351. |
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| Costa G, Lovegrove HE, Herbert SP. Endothelial cells divide unequally to sprout fairly, Cell Cycle 2017, 16:1-2. |
| Last Update |
| 2017-10-19 15:38:40 |
| The responsibility for this page contents is entirely of the student/alumnus. |
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Program financially supported by
the National Foundation for
Science and Technology
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