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| Name |
| Moura Alves, Pedro |
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| Nationality |
| Portuguese |
| E-Mail |
| mindgames81@gmail.com |
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| 1st Degree |
| Biochemistry |
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| University (1st Degree) |
| UBI |
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| About the PhD |
| Field of Research |
| Innate Immunity |
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| Thesis Title |
| Systematic identification of splicing factors with a role in IL-1β secretion by shRNA screening |
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| Supervisor(s) |
| Luis Filipe Ferreira Moita, M.D., PhD. |
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| University |
| Faculdade de Medicina Univesidade de Lisboa |
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| Laboratory |
| UBCSI-Instituto de Medicina Molecular |
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| City |
| Lisbon |
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| Country |
| Portugal |
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| Date of Thesis Defence |
| 2010-10-25 |
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| After the PhD (Current Situation) |
| Position |
| Postdoctoral Fellow |
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| Project |
| Immune responses to Mtb infection |
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| Institution |
| Max Planck Institute for Infection Biology |
| View Institution website |
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| City |
| Berlin |
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| Country |
| Germany |
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| Relevant Publications |
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Cell Microbiol. 2010 Aug;12(8):1046-63. Epub 2010 Feb 9.
Mycobacterium tuberculosis protein ESAT-6 is a potent activator of the NLRP3/ASC inflammasome.
Bibhuti B. Mishra1,†, Pedro Moura-Alves2,3,‡, Avinash Sonawane4, Nir Hacohen5,6, Gareth Griffiths4, Luis F. Moita2,*, Elsa Anes1,*
1Centro de Patogénese Molecular, Unidade dos Retrovírus e Infecções Associadas e Instituto de Medicina Molecular, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal.
2Cell Biology of the Immune System Unit, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal.
3Graduate Program in Basic and Applied Biology, Abel Salazar Institute of Biomedical Sciences, University of Porto, 4099-003 Porto, Portugal.
4Department of Molecular Biosciences, University of Oslo, PO Box 1041, Blindern, 0316 Oslo, Norway.
5Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital, Charlestown, MA 02129, USA.
6Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
*Correspondence: Luis F. Moita,
*Correspondence: Elsa Anes,
*Correspondence:
E-mail lmoita@fm.ul.pt; Tel. (+35) 121 799 9512; Fax (+35) 121 799 9504;
E-mail eanes@ff.ul.pt; Tel. (+35) 121 794 6443; Fax (+35) 121 793 4212.
‡These authors contributed equally to this work.
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| View Publication |
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An shRNA-based screen of splicing regulators identifies SFRS3 as a negative regulator of IL-1β secretion.
Moura-Alves P, Neves-Costa A, Raquel H, Pacheco TR, D'Almeida B, Rodrigues R, Cadima-Couto I, Chora Â, Oliveira M, Gama-Carvalho M, Hacohen N, Moita LF.
Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.
Abstract
The generation of diversity and plasticity of transcriptional programs are key components of effective vertebrate immune responses. The role of Alternative Splicing has been recognized, but it is underappreciated and poorly understood as a critical mechanism for the regulation and fine-tuning of physiological immune responses. Here we report the generation of loss-of-function phenotypes for a large collection of genes known or predicted to be involved in the splicing reaction and the identification of 19 novel regulators of IL-1β secretion in response to E. coli challenge of THP-1 cells. Twelve of these genes are required for IL-1β secretion, while seven are negative regulators of this process. Silencing of SFRS3 increased IL-1β secretion due to elevation of IL-1β and caspase-1 mRNA in addition to active caspase-1 levels. This study points to the relevance of splicing in the regulation of auto-inflammatory diseases. |
| View Publication |
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Program financially supported by
the National Foundation for
Science and Technology
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