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Class of 2004
Name
Moura Alves, Pedro
Nationality
Portuguese
E-Mail
mindgames81@gmail.com
1st Degree
Biochemistry
University (1st Degree)
UBI
About the PhD
Field of Research
Innate Immunity
Thesis Title
Systematic identification of splicing factors with a role in IL-1β secretion by shRNA screening
Supervisor(s)
Luis Filipe Ferreira Moita, M.D., PhD.
University
Faculdade de Medicina Univesidade de Lisboa
Laboratory
UBCSI-Instituto de Medicina Molecular
City
Lisbon
Country
Portugal
Date of Thesis Defence
2010-10-25
After the PhD (Current Situation)
Position
Postdoctoral Fellow
Project
Immune responses to Mtb infection
Institution
Max Planck Institute for Infection Biology
View Institution website
City
Berlin
Country
Germany

Relevant Publications
Cell Microbiol. 2010 Aug;12(8):1046-63. Epub 2010 Feb 9.

Mycobacterium tuberculosis protein ESAT-6 is a potent activator of the NLRP3/ASC inflammasome.
Bibhuti B. Mishra1,†, Pedro Moura-Alves2,3,‡, Avinash Sonawane4, Nir Hacohen5,6, Gareth Griffiths4, Luis F. Moita2,*, Elsa Anes1,*

1Centro de Patogénese Molecular, Unidade dos Retrovírus e Infecções Associadas e Instituto de Medicina Molecular, Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal.
2Cell Biology of the Immune System Unit, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1649-028 Lisboa, Portugal.
3Graduate Program in Basic and Applied Biology, Abel Salazar Institute of Biomedical Sciences, University of Porto, 4099-003 Porto, Portugal.
4Department of Molecular Biosciences, University of Oslo, PO Box 1041, Blindern, 0316 Oslo, Norway.
5Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital, Charlestown, MA 02129, USA.
6Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
*Correspondence: Luis F. Moita,

*Correspondence: Elsa Anes,

*Correspondence:

E-mail lmoita@fm.ul.pt; Tel. (+35) 121 799 9512; Fax (+35) 121 799 9504;

E-mail eanes@ff.ul.pt; Tel. (+35) 121 794 6443; Fax (+35) 121 793 4212.


‡These authors contributed equally to this work.
View Publication
An shRNA-based screen of splicing regulators identifies SFRS3 as a negative regulator of IL-1β secretion.

Moura-Alves P, Neves-Costa A, Raquel H, Pacheco TR, D'Almeida B, Rodrigues R, Cadima-Couto I, Chora Â, Oliveira M, Gama-Carvalho M, Hacohen N, Moita LF.

Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.

Abstract

The generation of diversity and plasticity of transcriptional programs are key components of effective vertebrate immune responses. The role of Alternative Splicing has been recognized, but it is underappreciated and poorly understood as a critical mechanism for the regulation and fine-tuning of physiological immune responses. Here we report the generation of loss-of-function phenotypes for a large collection of genes known or predicted to be involved in the splicing reaction and the identification of 19 novel regulators of IL-1β secretion in response to E. coli challenge of THP-1 cells. Twelve of these genes are required for IL-1β secretion, while seven are negative regulators of this process. Silencing of SFRS3 increased IL-1β secretion due to elevation of IL-1β and caspase-1 mRNA in addition to active caspase-1 levels. This study points to the relevance of splicing in the regulation of auto-inflammatory diseases.
View Publication
INSTITUTIONS INVOLVED
Program financially supported by
the National Foundation for
Science and Technology